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3.3 Prerequisites for First Use ​

Before starting, prepare BioLine access, fkit, input data, and a host environment that meets the selected Tool’s resource requirements.

  • An upstream Flow requires a valid BioLine account and a readable data location. fkit manages credentials; do not write them into task directories.
  • Downstream tasks require a compatible local container runtime that can run offline, mount stage read-only, and write task outputs.
  • Local storage is needed for the exact Tool version pulled for this task, staged inputs, and results. The historical image set does not need to be downloaded in advance.
  • CPU, memory, disk, and GPU requirements come from the selected Tool’s config/summary and the host runtime. Use a GPU only when that exact Tool supports it and the host is compatible.
  • Input formats depend on the objective and the discovered Tool/Flow interface. Common inputs include paired-end FASTQ, abundance or pathway tables, ASV tables, and metadata.tsv.
RequirementPurpose
fkit and a BioLine accountDiscover, inspect, and run platform Flows, Jobs, and Tools.
Container runtimeRun downstream task scripts within the restricted local boundary.
Available disk and computeHold this task’s exact Tool version, inputs, and outputs.

There is no need to keep a fixed historical image set locally. Discover existing BioLine Tools and Flows first, then inspect the exact version, documentation, and resource configuration. If no suitable capability exists, create one on demand.